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current
position > Education on plants > Botanical tissue culture
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| Seed
Exchange List /
Seed application
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| 仩Present
situation & Outline |
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[Purpose]
To preserve plant resources by reproducing plants that
are rare or in danger of extinction.
To put on display plants of great horticultural value. |
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[Details]
1. Developing and Cultivating plants of scarcity value.
2. Reproducing plants that are of greate horticultural
value and popular among the visitors.
3. Reproducing plants that need to be increased in their
number for the exhibition in the botanical garden.
4. Reproducing and Cultivating plants that are protected
by the related law and plants that Korea Forest Service
designates as rare plants or as plants in danger of extinction.
6. Habitat restoration and Up-to-date Education on plants.
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[Facilities for Cultivation] |
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1pyong equivalent to about
3.3 |
Items
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Workroom
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Germ-free Room
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Cultivating Room
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Growing Land |
Area
| 8 
| 3 
| 13
| 100 |
kind of work
| Making Reagent Washing
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Germ-free Works (seeding,
transplantation)
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Seeding Culture
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Growing Cultivated plants |
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What is Tissue culture(:In vitro vulture)? |
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[Characteristics & Purpose
of Culture] |
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| Plant medium culture could be understood as
a method in which we can grow plarts of plants,
such as seeds, embryos, organs, cells or protoplast,
in a medium that is conditioned with needed nutritions
under a germ-free condition. According to which
part of a plant is used, the plant medium cultures
are divided into embryo culture, organ culture,
cell culture, protoplast culture and pollen culture,
etc. Since its development in the 19th century,
this |

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| relatively new kind of reproduction
method has been gradually advanced until now.
In fact, the fields of its use also have been
expanded, being used in such fields as horticulture,
agriculture, food, medicine, and so on.
Compared with conventional ways, plant medium
culture requires much less space to grow plants
in, and it cuts the cost greatly. These facts
allow us to have more price competitiveness.
As the tissue culture methods are advanced, it
has been much easier to obtain somatic hybrids,
sexual reproductions, or quasi-sexual reproductions
by regenerating a complete plant from protoplast
or cell of a plant.
When we use these kinds of culture methods, as
in protoplast culture, we could overcome the genetic
barriers to make new types of plant individual.
Plant breeders, geneticists, molecular biologists
and other scientists are conducting studies related
with various culture methods.
A lot of studies on these methods, such as those
which apply o medicine, the second metabolite
extracted from plant or animal tissue, are being
conducted.
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[Culture Medium] |
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| In 1962, Murashige and Skoog developed a medium
for plant tissue culture, and it is called MS medium.
MS medium is made of compound of mineral salt, various
vitamins and other energy resources and it is used
most frequently as a basic medium. Almost 100 years
of tissue culture history has continuously caused
us to develop a medium proper to each plant. Higher
plant life needs 9 essential elements (C, H, O,
N, P, K, S, Ca, Mg) |
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in great quantity and 7 essential
elements (Fe, Mn, Cu, Zn, Mo, B, Cl) in small quantity.
Plants can be provided with essential elements,
various vitamins, and energy resources and when
needed, the growth regulator or other additives
(for example, gelatin or activated carbon) can be
supplied in accordance with the need of each plant. |
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