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Botanical tissue culture
Interchange of seed
current position > Education on plants > Botanical tissue culture
 
   
 
 
 
Seed Exchange List   /    Seed application
Present situation & Outline
  [Purpose]
To preserve plant resources by reproducing plants that are rare or in danger of extinction.
To put on display plants of great horticultural value.

  [Details]
1. Developing and Cultivating plants of scarcity value.
2. Reproducing plants that are of greate horticultural value and popular among the visitors.
3. Reproducing plants that need to be increased in their number for the exhibition in the botanical garden.
4. Reproducing and Cultivating plants that are protected by the related law and plants that Korea Forest Service designates as rare plants or as plants in danger of extinction.
6. Habitat restoration and Up-to-date Education on plants.

 
  [Facilities for Cultivation]
 
1pyong equivalent to about 3.3
Items
Workroom
Germ-free Room
Cultivating Room
Growing Land
Area
8
3
13
100
kind of work
Making Reagent Washing
Germ-free Works (seeding,
transplantation)
Seeding Culture
Growing Cultivated plants
 
What is Tissue culture(:In vitro vulture)?
  [Characteristics & Purpose of Culture]
 

Plant medium culture could be understood as a method in which we can grow plarts of plants, such as seeds, embryos, organs, cells or protoplast, in a medium that is conditioned with needed nutritions under a germ-free condition. According to which part of a plant is used, the plant medium cultures are divided into embryo culture, organ culture, cell culture, protoplast culture and pollen culture, etc. Since its development in the 19th century, this

relatively new kind of reproduction method has been gradually advanced until now. In fact, the fields of its use also have been expanded, being used in such fields as horticulture, agriculture, food, medicine, and so on.
Compared with conventional ways, plant medium culture requires much less space to grow plants in, and it cuts the cost greatly. These facts allow us to have more price competitiveness.
As the tissue culture methods are advanced, it has been much easier to obtain somatic hybrids, sexual reproductions, or quasi-sexual reproductions by regenerating a complete plant from protoplast or cell of a plant.

When we use these kinds of culture methods, as in protoplast culture, we could overcome the genetic barriers to make new types of plant individual. Plant breeders, geneticists, molecular biologists and other scientists are conducting studies related with various culture methods.
A lot of studies on these methods, such as those which apply o medicine, the second metabolite extracted from plant or animal tissue, are being conducted.

 
 
  [Culture Medium]
 
In 1962, Murashige and Skoog developed a medium for plant tissue culture, and it is called MS medium. MS medium is made of compound of mineral salt, various vitamins and other energy resources and it is used most frequently as a basic medium. Almost 100 years of tissue culture history has continuously caused us to develop a medium proper to each plant. Higher plant life needs 9 essential elements (C, H, O, N, P, K, S, Ca, Mg)
in great quantity and 7 essential elements (Fe, Mn, Cu, Zn, Mo, B, Cl) in small quantity.
Plants can be provided with essential elements, various vitamins, and energy resources and when needed, the growth regulator or other additives (for example, gelatin or activated carbon) can be supplied in accordance with the need of each plant.

 

 

 
  Saekdal-dong Seogwipo Si JEJU-DO 2920 KR (697-808) / Tel : 82-64-735-1100 Fax : 82-64-73-2992
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